Indian Journal of Pharmacy and Pharmacology

Print ISSN: 2393-9079

Online ISSN: 2393-9087

CODEN : IJPPTK

Indian Journal of Pharmacy and Pharmacology (IJPP) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, more...


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Original Article


Article page

160-168


Authors Details

Mohan Gandhi B, Lakshmana Rao A, Venkateswara Rao J


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Development and Validation of Spectrophotometric and Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Gatifloxacin and Flubiprofen Sodium in Ophthalmic Dosage Form


Original Article

Author Details : Mohan Gandhi B, Lakshmana Rao A, Venkateswara Rao J

Volume : 2, Issue : 3, Year : 2015

Article Page : 160-168


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Abstract

Objective: The main objective of this work is to develop simple, precise, accurate and reproducible UV-Spectrophotometric and Stability indicating RP-HPLC methods for simultaneous estimation of Gatifloxacin (GAT) and Flubiprofen sodium (FLU) in ophthalmic dosage form.
Methods: Dual wavelength spectrophotometric method which involves solving of simultaneous equations based on the measurement of absorbances at 289 nm and 248 nm, which are the absorption maxima (?max) of GAT and FLU respectively. The RP-HPLC analysis is carried out on Shiseido C18 column (250 mm × 4.6 mm I.D.), using 1% orthophosphoric acid in water and acetonitrile in the ratio of (40:60 % v/v) as the mobile phase with a   flow rate of 0.9 ml/min. The detection was carried out at a wavelength of 236 nm.
Results: The retention times were found to be 2.152±0.2 min and 7.881±0.2 min for GAT and FLU respectively. The linearity range was found to be 10-20 ?g/ml and 1-2 ?g/ml for Gatifloxacin and Flubiprofen sodium respectively by UV method and 10-30 ?g/ml and 1-3 ?g/ml for Gatifloxacin and Flubiprofen sodium respectively by HPLC method. The percentage recoveries of both the drugs GAT and FLU from the ophthalmic form were 99.51% and 99.58% respectively by UV method and 99.08% and 99.43% respectively by HPLC method. The correlation coefficients of both the drugs were found to be more than 0.99 by two methods. Other parameters like ruggedness, robustness etc. was well within the acceptance criteria.
Conclusion: Both UV-spectrophotometric and stability indicating RP-HPLC methods were found to be accurate, rapid, precise and simple. These simple methods can be used for the simultaneous estimation of GAT and FLU in bulk and in ophthalmic dosage forms.

Keywords: Gatifloxacin, Flubiprofen sodium, Simultaneous equation, Validation, RP-HPLC.


How to cite : Mohan Gandhi B, Lakshmana Rao A, Venkateswara Rao J, Development and Validation of Spectrophotometric and Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Gatifloxacin and Flubiprofen Sodium in Ophthalmic Dosage Form. Indian J Pharm Pharmacol 2015;2(3):160-168

Copyright © 2015 by author(s) and Indian J Pharm Pharmacol. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (creativecommons.org)







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