Indian Journal of Pharmacy and Pharmacology

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Indian Journal of Pharmacy and Pharmacology (IJPP) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, more...


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Udyavar, Kumar K, Rai, Gopalakrishna, Chandrashekar R, and Sowmya: Evaluation of antidepressant activity of ethanolic extract of Mimosa pudica in swiss albino mice


Introduction

Depression is an extremely prevalent form of mental illnesses worldwide. In 2017, World Health Organisation released a report which estimated that 4.4% of the global population suffered from depression.1 Depressed patients commonly complain about feeling sad, lack of interest in their day to day work, inability to find pleasure in activities that would normally please others, and feelings of discontent. It is also associated with feeling of guilt or reduced self-worth, disturbed sleep, changes in appetite, and low energy.2 In severe forms of depression, patients are known to cause self harm or even suicide.3 Although drugs available for the therapy of depression, they have their limitations, such as delayed therapeutic response and low responders to these drugs, which poses a problem with patient compliance.4, 5 Hence, there is a requirement for alternative treatment of depressive disorders with the use of medicinal plants.

The plant Mimosa pudica is a weed that grows in humid areas, open fields and by roadsides. It grows as a shrub, under 100 cm in height, and is easily identifiable by its characteristic 15 – 20 pairs of leaflets that folds when disturbed, and is hence known as “Lajwanti” in Hindi, and “Touch me not plant” in English.6 It is believed to be native to the Middle Americas and is now found in other in all tropical countries of the Asian subcontinent and South East Asia.7 It has been used as a folk lore medicine since many years because of its various medicinal properties, and because it is an easily cultivable plant and is abundantly available.8

It has been used in treatment of hemorrhoids, anal fistulas, diarrhea and dysentery. The plant roots were used in treatment of respiratory conditions like cough and asthma, and in some urinary infections.9 Phytochemical studies revealed the presence of alkaloids, amino acid, flavonoids glycosides, sterols, terpenoids, tannins and fatty acids in this plant.10

The purpose of this study was to get a better understanding of the antidepressant activity of Mimosa pudica.

Materials and Methods   

Ethical clearance was obtained from the Institutional Animal Ethics Committee before starting the study (Ref No. IAEC/02/02/2019/CPCSEA).

Swiss albino mice, 60 in number, weighing 20-30 g, of either sex, maintained under standard conditions in the Institutional animal house were used. They were housed in clean, transparent polypropylene cages in groups of six and maintained at standard laboratory temperature and humidity (40-60%) with light/dark cycle of 12:12 hours. Animals were fed commercial pelleted chow and water. The mice were allowed to acclimatize to these conditions for a week before starting the experiments.

The standard drug, Imipramine hydrochloride, was obtained from Abbot healthcare Pvt Ltd (Depsonil 25). Mimosa pudica plant was obtained from district Udupi, Karnataka, India. The plant was authenticated by Jyothi K T, Lecturer and HOD, Department of Botany, Sri Siddhartha First Grade College, Tumkur. Ethanol 99.9% was obtained from Changshu Yanguan Chemicals.

The Mimosa pudica plants were washed, the leaves were shade dried and powdered. About 200 g of the dried leaf powder of Mimosa pudica was extracted with 99.9% Ethanol in Soxhlet extractor for about 36 hours. The ethanol was then evaporated from the mixture by placing it in a beaker and heating it over a water bath. The extract gave a yield of brownish paste like mass weighing 6g. The yield obtained was 3% w/w with respect to dried powder.11

The mice (n=60) were divided into two arms which was further divided into five groups, each group having six mice. Drugs were given orally after 12 hours of fasting every day, for ten days.

The drugs were prepared and administered per oral (0.1ml/10g).

Group 1 was administered normal saline(10ml/kg).

Group 2 was given standard drug Imipramine (15mg/kg).12

Group 3, 4 and 5 received 100mg/kg, 200mg/kg, 400mg/kg doses of the test compound Ethanolic Extract of Mimosa pudica (EEMP) respectively.13

For the Acute study, on day 1, one arm of 30 mice were subjected to Tail Suspension Test (TST), while 30 mice in the other arm were subjected to Forced Swim Test (FST), one hour after feeding the respective drugs. For Subacute study, on day 10, the mice were again subjected to TST and FST, one hour after feeding respective drugs.

Procedure

Forced swim test (FST)

The method used was as described by Porsolt et al. The mice were induvidually forced to swim in a vertical plexiglass cylinder (capacity: 5L, height: 50cm diameter: 18cm) containing l5cm of water maintained at temperature: 25°C. Mice were subjected to pre-screening, which lasted for 15 minutes. 24 hours after pre-screening, the trial was performed for 6 minutes of which the first two minutes were not recorded, and the periods of immobility for the latter four minutes was measured (in seconds) with a stopwatch. Mice were considered to be immobile when they made only the bare necessary movements to stay afloat, or when they were motionless. The mice were taken out of the plexiglass cylinder after 6 minutes. They were dried with a dry towel, and kept under a dim lamp for drying. The water was discarded after every test, and fresh water was used for the next mouse.14, 15

Tail suspension test (TST)

The method used was as described by Steru et al. Antidepressants that are used in practice are able to reduce the period of immobility of mice when they try to escape when suspended by their tail. This test was a reliable screening method for antidepressants, including those involving serotonergic system. Mice ware hung on a wooden rod, 50 cm above the table, by attaching them from their tail end with the use of an adhesive tape. The first two minutes were not recorded, and the periods of immobility for the latter six minutes was recorded (in seconds) with a stopwatch. Mice were considered to be immobile only when they were motionless and not attempting to escape.14, 16

Statistics

The recorded data was entered in Microsoft Excel.

The variables recorded followed normal distribution, hence, results have been expressed as mean (in seconds) ± standard error of mean (SEM). The data was analysed using one way ANOVA followed by post-hoc Dunnet’s test.

Probability ‘p’ value less than 0.05 was considered as statistically significant.

Table 1

Day 1

Tail Suspension Test

Forced Swim Test

Normal Saline

231.3(±19.55)

139.5(±6.43)

Imipramine 15mg/kg

175.3(±5.34)*

104.83(±5.86)*

EEMP 100mg/Kg

169.7(±13.8)*

94.83(±7.67)*

EEMP 200mg/kg

181.7(±9.81)*

111.83(±3.00)*

EEMP 400mg/kg

116.8(±6.65)*

109.16(±5.93)*

Table 2

Day 10

Tail Suspension Test

Forced Swim test

Normal Saline

225.16(±12.32)

144(±5.49)

Imipramine 15mg/kg

151.33(±11.64)*

92.17(±7.00)*

EEMP 100mg/Kg

93.66(±8.196)*

68.667(±8.58)*

EEMP 200mg/kg

211.33(±16.77)#

91.667(±15.38)*

EEMP 400mg/kg

102(±14.74)*

86.5(±6.386)*

Figure 1
https://typeset-prod-media-server.s3.amazonaws.com/article_uploads/b1fa0512-fa3b-48c8-bb1f-c3a4791ef8b0/image/031110ca-f186-4fbb-82c3-6552e4ed7a21-uimage.png

Figure 2
https://typeset-prod-media-server.s3.amazonaws.com/article_uploads/b1fa0512-fa3b-48c8-bb1f-c3a4791ef8b0/image/5f2c24cc-9147-4909-9a3f-4ae44c72f7fe-uimage.png

Results

In the Acute study, on Day 1, standard drug Imipramine (15mg/kg) and test drug EEMP (100mg/kg, 200mg/kg, 400mg/kg) showed significant reduction in immobility times when compared to control in both FST and TST (Table 1, Figure 1). In the Subacute study, on Day 10, both Imipramine (15mg/kg) and EEMP (100mg/kg, 400mg/kg) showed significant reduction in immobility times when compared to control in both FST and TST (Table 2, Figure 2).

Discussion

A previous study concluded that lyophilized aqueous leaf extract of Mimosa pudica exhibited antidepressant and anxiolytic properties in forced swimming (FST) and tail suspension (TST) tests, the elevated plus maze (EPM) model and locomotor activity count.17 Another study on Effects of Mimosa pudica leaves extract showed that ethyl acetate extract of Mimosa pudica has anti-anxiety, anti-depressant and memory enhancing activities.13

In this study, both Imipramine and EEMP showed a reduction in immobility times in acute and subacute study in both FST and TST. Lowest immobility times were recorded with EEMP at 100 mg/kg doses in most recordings, and at times, it showed comparable or even better reduction in immobility times than Imipramine in both tests in acute and subacute study.

Imipramine inhibits Norepinephrine transporter and Serotonin transporters, increasing their availability at synaptic cleft, thereby reducing depression. The anti-depressant action of EEMP is probably similar to the mechanisms of anti-depressant agents, like Imipramine, that are effective in the above screening models.

Phytochemical investigations done in a study showed the presence of alkaloids, flavonoids and tannins in the extract. It is likely that the antidepressant activity seen with Mimosa pudica could be because of the above mentioned phyto-constituents.18 Further pharmacological investigations are required to understand its underlying mechanism of action in depression.

Conclusion

The results obtained in this study suggests that Ethanolic Extract of Mimosa pudica has anti-depressant activity and can be considered for use in therapy of depression after further testing.

Source of Funding

None.

Conflicts of Interest

Nil.

References

1 

UN News, UN health agency reports depression now ‘leading cause of disability worldwide’https://news.un.org/en/story/2017/02/552062-un-health-agency-reports-depression-now-leading-cause-disability-worldwide

2 

National Institute of Mental Health. Depression Basics, [Internet]https://www.nimh.nih.gov/health/publications/depression/index.shtml

3 

S Bachmann Epidemiology of Suicide and the Psychiatric PerspectiveInt J Environ Res Public Health20181571425

4 

R Machado-Vieira J Baumann C Wheeler-Castillo D Latov I Henter G Salvadore The Timing of Antidepressant Effects: A Comparison of Diverse Pharmacological and Somatic TreatmentsPharmaceuticals201031194110.3390/ph3010019

5 

PA Kudlow RS McIntyre RW Lam Early Switching Strategies in Antidepressant Non-Responders: Current Evidence and Future Research DirectionsCNS Drugs2014287601910.1007/s40263-014-0171-5

6 

Invasive Species Compendium. Mimosa pudica (sensitive plant), [Internet]https://www.cabi.org/isc/datasheet/34202

8 

R Gupta H Ahmad S Sehgal A Mishra Mimosa pudica L. (Laajvanti): An overviewPharmacognosy Rev20126121152410.4103/0973-7847.99945

9 

CP Khare Encyclopedia of Indian Medicinal PlantsSpinger PublicationGermany20043134

10 

PK Vikram R Malvi DK Jain Evaluation of Analgesic and Anti-Inflammatory Potential of Mimosa Pudica LinnInt J Curr Pharm Res2012444750

11 

M Khan N Harun AHN Ab Rehman SAA Elhussein In Vitro Antioxidant Evaluation of Extracts of Three Wild Malaysian PlantsProcedia Engg201353293610.1016/j.proeng.2013.02.005

12 

M Chowta G Pallempati A Rai A Singh A Shoeb Evaluation of antidepressant activity of vanillin in miceIndian J Pharmacol2013452141410.4103/0253-7613.108292

13 

G Patro S K Bhattamisra B K Mohanty Effects of Mimosa pudica L. leaves extract on anxiety, depression and memoryAvicenna J Phytomed201666696710

14 

GH Vogel Drug discovery and evalution-pharmacological assaysSpinger PublicationGermany200778893

15 

RD Porsolt A Bertin M Jalfre Behavioral despair in mice: A primary screening test for antidepressantsArch Int Pharmacodyn Ther197722932736

16 

L Steru R Chermat B Thierry P Simon The tail suspension test: A new method for screening antidepressants in micePsychopharmacol19858533677010.1007/bf00428203

17 

G De Guzman M Ysrael A Dacanay GJ Alejandro S Valentin The anti-depressant and anxiolytic properties of the lyophilized aqueous leaf extract of Mimosa pudica L. (Fabaceae)Res J Phytomed2015113942

18 

I Sajid BK Karmaker Z Rashid M Islam ME Haque CNS Depressant and Antinociceptive Activities of the Aerial Parts of Mimosa pudicaEur J Appl Sci20135412733



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Article type

Original Article


Article page

240-244


Authors Details

Sanat Udyavar, Sharath Kumar K, Mohandas Rai, H N Gopalakrishna, Chandrashekar R, Sowmya


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